NDLI: Detection of Tick-Borne Pathogens in Red Deer (Cervus elaphus), United Kingdom

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Detection of Tick-Borne Pathogens in Red Deer (Cervus elaphus), United Kingdom

Content Provider	MDPI
Author	Johnson, Nicholas Golding, Megan Phipps, Laurence
Copyright Year	2021
Description	Deer represent a major vertebrate host for all feeding stages of the hard tick Ixodes ricinus in the United Kingdom (UK), and could play a role in the persistence of tick-borne pathogens. However, there have been few studies reporting the presence of Babesia spp. and Anaplasma phagocytophilum in deer in the UK, and those that detected Babesia were unable to confirm the species. To address this, we have investigated blood samples from red deer (Cervus elaphus) for the presence of tick-borne pathogens. Total DNA was extracted from haemolysed blood that was removed from clotted blood sampled from culled, captive red deer. Babesia spp. were detected with a pan-piroplasm PCR that amplifies a fragment of the 18S rRNA gene. Species were identified based on identity with published sequences. Anaplasma phagocytophilum was detected with a probe-based PCR targeting the msp2 gene. In addition, residual serum samples from a subset of animals were tested for the presence of anti-flavivirus antibodies. Of 105 red deer samples tested from three locations in the United Kingdom, 5 were positive for piroplasm and 5 were positive for A. phagocytophilum. Co-infection with both pathogens was detected in two samples from one location. No evidence for antibodies against West Nile virus were detected. However, 12% of sera tested were positive for tick-borne encephalitis virus antibodies.
Starting Page	640
e-ISSN	20760817
DOI	10.3390/pathogens10060640
Journal	Pathogens
Issue Number	6
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-05-23
Access Restriction	Open
Subject Keyword	Pathogens Parasitology Tropical Medicine Babesia Anaplasma Phagocytophilum Red Deer 18s Rrna Gene Flavivirus
Content Type	Text
Resource Type	Article

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