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Cultivation of Chlorella sorokiniana IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale
| Content Provider | MDPI |
|---|---|
| Author | Gabrielyan, David A. Sinetova, Maria A. Gabel, Boris V. Gabrielian, Alexander K. Markelova, Alexandra G. Rodionova, Margarita V. Bedbenov, Vladimir S. Shcherbakova, Natalia V. Los, Dmitry A. |
| Copyright Year | 2022 |
| Description | Flat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae Chlorella sorokiniana IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, C. sorokiniana was grown aseptically in 250 mL glass vessels; then, it was diluted and inoculated into a 5-liter flat-panel horizontal photobioreactor; and, at the last stage, the culture was diluted and inoculated into a 70-liter flat-panel vertical photobioreactor. In the presented cycle, the cultured biomass increased by 326 times in 13 days (from 0.6 to 195.6 g dw), with a final biomass concentration of 2.8 g dw $L^{−1}$. The modes of semi-continuous cultivation were considered. The biomass harvest and dilution of the suspension were carried out either every day or every 3–4 days. For C. sorokiniana IPPAS C-1, a conversion coefficient of optical density values to dry biomass (g $L^{−1}$) was refined through a factor of 0.33. The key parameters of the photobioreactors tested in this work are discussed. |
| Starting Page | 1309 |
| e-ISSN | 20751729 |
| DOI | 10.3390/life12091309 |
| Journal | Life |
| Issue Number | 9 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-08-25 |
| Access Restriction | Open |
| Subject Keyword | Life Microbiology Chlorella Biomass Cultivation Flat-panel Photobioreactor Microalgae |
| Content Type | Text |
| Resource Type | Article |
