NDLI: Extracellular DNA: A Critical Aspect of Marine Biofilms

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Extracellular DNA: A Critical Aspect of Marine Biofilms

Content Provider	MDPI
Author	Tuck, Benjamin Salgar-Chaparro, Silvia J. Watkin, Elizabeth Somers, Anthony Forsyth, Maria Machuca, Laura L.
Copyright Year	2022
Description	Multispecies biofilms represent a pervasive threat to marine-based industry, resulting in USD billions in annual losses through biofouling and microbiologically influenced corrosion (MIC). Biocides, the primary line of defence against marine biofilms, now face efficacy and toxicity challenges as chemical tolerance by microorganisms increases. A lack of fundamental understanding of species and EPS composition in marine biofilms remains a bottleneck for the development of effective, target-specific biocides with lower environmental impact. In the present study, marine biofilms are developed on steel with three bacterial isolates to evaluate the composition of the EPSs (extracellular polymeric substances) and population dynamics. Confocal laser scanning microscopy, scanning electron microscopy, and fluorimetry revealed that extracellular DNA (eDNA) was a critical structural component of the biofilms. Parallel population analysis indicated that all three strains were active members of the biofilm community. However, eDNA composition did not correlate with strain abundance or activity. The results of the EPS composition analysis and population analysis reveal that biofilms in marine conditions can be stable, well-defined communities, with enabling populations that shape the EPSs. Under marine conditions, eDNA is a critical EPS component of the biofilm and represents a promising target for the enhancement of biocide specificity against these populations.
Starting Page	1285
e-ISSN	20762607
DOI	10.3390/microorganisms10071285
Journal	Microorganisms
Issue Number	7
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2022-06-24
Access Restriction	Open
Subject Keyword	Microorganisms Environmental Engineering Extracellular Dna Microbiologically Influenced Corrosion Biofilm Extracellular Polymeric Substances Epss
Content Type	Text
Resource Type	Article

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