NDLI: Characterization of Xanthomonas arboricola pv. juglandis Bacteriophages against Bacterial Walnut Blight and Field Evaluation

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Characterization of Xanthomonas arboricola pv. juglandis Bacteriophages against Bacterial Walnut Blight and Field Evaluation

Content Provider	MDPI
Author	Pablo, Núñez Retamales, Julio Alvarado, Romina Campan, Erick D. M. Otto, Thierry Segovia, Cristopher Vasquez, Ignacio Santander, Javier
Copyright Year	2022
Description	Xanthomonas arboricola pv. juglandis (hereafter X. juglandis) is the etiological agent of walnut blight, the most important bacterial disease affecting walnut production worldwide. Currently, the disease is treated mainly with copper-derived compounds (e.g., $CuSO_{4}$) despite the evidence of genetic resistance in these strains. Regarding the effectiveness and sustainability, the use of a bacteriophage appears to be a biocontrol alternative to reduce X. juglandis load and symptomatology of walnut blight. Here, the phages f20-Xaj, f29-Xaj, and f30-Xaj were characterized, and their effectiveness in walnut orchards against walnut blight was determined. These bacteriophages showed a specific lytic infection in X. juglandis strains isolated from Chile and France. Phylogenetic analysis of the complete genome of f20-Xaj and f30-Xaj indicates that these phages belong to the Pradovirus genus. In the field, the cocktail of these bacteriophages showed similar effectivity to $CuSO_{4}$ in the reduction of incidence and severity in walnut tissue. Moreover, the bacterial load of X. juglandis was significantly reduced in the presence of bacteriophages in contrast to a $CuSO_{4}$ treatment. These results show that the use of bacteriophages can be an alternative to combat the symptoms of walnut blight caused by X. juglandis.
Starting Page	1380
e-ISSN	19994915
DOI	10.3390/v14071380
Journal	Viruses
Issue Number	7
Volume Number	14
Language	English
Publisher	MDPI
Publisher Date	2022-06-24
Access Restriction	Open
Subject Keyword	Viruses Entomology Xanthomonas Arboricola Pv. Juglandis Phage Therapy Walnut Blight
Content Type	Text
Resource Type	Article

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