NDLI: Phenolic Profile, Antioxidant Capacity and Antimicrobial Activity of Nettle Leaves Extracts Obtained by Advanced Extraction Techniques

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Phenolic Profile, Antioxidant Capacity and Antimicrobial Activity of Nettle Leaves Extracts Obtained by Advanced Extraction Techniques

Content Provider	MDPI
Author	Ivona, Elez Garofulić Maja, Repajić Zoran, Zorić Sandra, Pedisić Meta, Sterniša Sonja, Smole Možina Verica, Dragović-Uzelac Malin, Valentina
Copyright Year	2021
Description	Nettle is a widely known plant whose high biological activity and beneficial medicinal effects are attributed to various bioactive compounds, among which polyphenols play an important role. In order to isolate polyphenols and preserve their properties, advanced extraction techniques have been applied to overcome the drawbacks of conventional ones. Therefore, microwave-assisted extraction (MAE) has been optimized for the isolation of nettle leaves polyphenols and it was compared to pressurized liquid extraction (PLE) and conventional heat-reflux extraction (CE). The obtained extracts were analyzed for their individual phenolic profile by UPLC $MS^{2}$ and for their antioxidant capacity by ORAC assay. MAE proved to be the more specific technique for the isolation of individual phenolic compounds, while PLE produced extracts with higher amount of total phenols and higher antioxidant capacity. Both techniques were more effective compared to CE. PLE nettle extract showed antimicrobial activity against bacteria, especially against Gram-negative Pseudomonas fragi ATCC 4973 and Campylobacter jejuni NCTC 11168 strains. This suggests that PLE is suitable for obtaining a nettle extract with antioxidant and antimicrobial potential, which as such has great potential for use as a value-added ingredient in the food and pharmaceutical industry.
Starting Page	6153
e-ISSN	14203049
DOI	10.3390/molecules26206153
Journal	Molecules
Issue Number	20
Volume Number	26
Language	English
Publisher	MDPI
Publisher Date	2021-10-12
Access Restriction	Open
Subject Keyword	Molecules Nettle Leaves Microwave-assisted Extraction Pressurized Liquid Extraction Advanced Extraction Techniques Plant Polyphenols Antioxidant Capacity Orac Uplc Ms2 Antimicrobial Activity
Content Type	Text
Resource Type	Article

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