NDLI: Fragrance in Pandanus amaryllifolius Roxb. Despite the Presence of a Betaine Aldehyde Dehydrogenase 2

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Fragrance in Pandanus amaryllifolius Roxb. Despite the Presence of a Betaine Aldehyde Dehydrogenase 2

Content Provider	MDPI
Author	Bhatt, Vacha Barvkar, Vitthal Furtado, Agnelo Henry, Robert Nadaf, Altafhusain
Copyright Year	2021
Description	Pandanus amaryllifolius Roxb. accumulates the highest concentration of the major basmati aroma volatile 2-acetyl-1-pyrroline (2AP) in the plant kingdom. The expression of 2AP is correlated with the presence of a nonfunctional betaine aldehyde dehydrogenase 2(BADH2) in aromatic rice and other plant species. In the present study, a full-length BADH2 sequence was reconstructed from the transcriptome data of leaf tissue from P. amaryllifolius seedlings. Based on this sequence, a 1509 bp coding sequence was defined that encoded a 54 kD PaBADH2 protein. This revealed the presence of a full-length BADH2 protein in P. amaryllifolius. Moreover, quantitative real-time PCR analysis, combined with BADH2 enzyme activity, confirmed the expression and functionality of the PaBADH2 protein. To understand the apparent structural variation, docking analysis was carried out in which protein showed a good affinity with both betaine aldehyde (BAD) and γ-aminobutyraldehyde (GAB-ald) as substrates. Overall, the analysis showed the presence of a functional BADH2, along with substantial 2AP synthesis (4.38 ppm). Therefore, we conclude that unlike all other plants studied to date, 2AP biosynthesis in P. amaryllifolius is not due to the inactivation of BADH2.
Starting Page	6968
e-ISSN	14220067
DOI	10.3390/ijms22136968
Journal	International Journal of Molecular Sciences
Issue Number	13
Volume Number	22
Language	English
Publisher	MDPI
Publisher Date	2021-06-28
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Plant Sciences P. Amaryllifolius Betaine Aldehyde Dehydrogenase 2 2-acetyl-1-pyrroline Γ-aminobutyraldehyde
Content Type	Text
Resource Type	Article

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