NDLI: Impact and Persistence of Serratia marcescens in Tenebrio molitor Larvae and Feed under Optimal and Stressed Mass Rearing Conditions

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Impact and Persistence of Serratia marcescens in Tenebrio molitor Larvae and Feed under Optimal and Stressed Mass Rearing Conditions

Content Provider	MDPI
Author	Dupriez, Florent Rejasse, Agnès Rios, Alfredo Lefebvre, Thomas LeRoux, Christina Nielsen-
Copyright Year	2022
Description	Industrial insect mass rearing aims to produce quality insects under safe sanitary conditions which can be compromised by pathogens and abiotic stressors. Therefore, knowledge on pathogen persistence, virulence and means of detection is of importance. This study focuses on the opportunistic pathogen Serratia marcescens (Sm) as a possible candidate to reveal sanitary issues in Tenebrio molitor (Tm) breeding. A screening test was performed to assess the impact of abiotic stressors (starvation, density and sieving) in presence and absence of Sm. Two Sm detection methods were conducted, and the kinetics of Sm persistence were investigated. Our results show that (i) the presence of Sm had a low but significant effect on Tm mortality, (ii) a short temporary starvation period had a negative impact on larval growth, (iii) the detection of Sm by q-PCR was sensitive but less convenient than a specific Sm growth media, (iv) the kinetics of persistence showed that Sm declined but survived for nine days in the feed and in the feces for three weeks. Both the relatively low virulence and the persistence in the environment suggest that Sm could be used as an indicator for the sanitary status of mealworm production.
Starting Page	458
e-ISSN	20754450
DOI	10.3390/insects13050458
Journal	Insects
Issue Number	5
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2022-05-12
Access Restriction	Open
Subject Keyword	Insects Entomology Food Safety Yellow Mealworm Serratia Marcescens Microbial Persistence Qpcr Insect for Food and Feed
Content Type	Text
Resource Type	Article

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