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Genetically Encoded Biosensors Based on Fluorescent Proteins
| Content Provider | MDPI |
|---|---|
| Author | Kim, Hyunbin Ju, Jeongmin Lee, Hae Nim Chun, Hyeyeon Seong, Jihye |
| Copyright Year | 2021 |
| Description | Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs. |
| Starting Page | 795 |
| e-ISSN | 14248220 |
| DOI | 10.3390/s21030795 |
| Journal | Sensors |
| Issue Number | 3 |
| Volume Number | 21 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-01-25 |
| Access Restriction | Open |
| Subject Keyword | Sensors Biochemistry and Molecular Biology Fluorescent Protein Genetically Encoded Biosensor Fret Ddfp Bifc Split Fp Circular Permutation Fluorescent Timer |
| Content Type | Text |
| Resource Type | Article |
