NDLI: Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of Brassica rapa L.

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Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of Brassica rapa L.

Content Provider	MDPI
Author	Cao, Wenyang Wang, Chenxi Mayhesumu, Xiayidan Pan, Le Dang, Yan Yili, Abulimiti Abuduwaili, Aytursun Mansur, Sanawar
Copyright Year	2022
Description	The aim of this study was to investigate the effects of microwave ultrasonic-assisted extraction (MUAE) on the content, structure, and biological functions of Brassica rapa L. polysaccharide (BRP). Response surface methodology (RSM) was used to optimize the parameters of MUAE, and it obtained a polysaccharide with yield of 21.802%. Then, a neutral polysaccharide named BRP-1-1 with a molecular weight of 31.378 kDa was isolated and purified from BRP using DEAE-650 M and Sephadex G-100. The structures of the BRP-1-1 were elucidated through a combination of FT-IR, GC-MS, NMR, and methylation analysis. The results showed that BRP-1 consisted of mannose (Man) and glucose (Glu) in a molar ratio of 7.62:1. The backbone of BRP-1-1 mainly consisted of →6)-α-D-Glup-(1→4-β-D-Glup-(1→2)-α-D-Manp-(1→2)-α-D-Glup-(1→, the branch was $[T-α-D-Manp-(1]_{n}$→. BRP-1-1 intervention significantly inhibited α-glucosidase activity; an inhibition rate of 44.623% was achieved at a concentration of 0.5 mg/mL. The results of the in vitro biological activity showed that BRP-1-1 has good antioxidant and hypoglycemic activity, suggesting that BRP-1-1 could be developed as a functional medicine.
Starting Page	3002
e-ISSN	14203049
DOI	10.3390/molecules27093002
Journal	Molecules
Issue Number	9
Volume Number	27
Language	English
Publisher	MDPI
Publisher Date	2022-05-07
Access Restriction	Open
Subject Keyword	Molecules Integrative and Complementary Medicine Brassica Rapa L. Muae Polysaccharides Antioxidant and Hypoglycemic Activities Dpph Α-glucosidase Α-amylase
Content Type	Text
Resource Type	Article

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