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Formation of β-Strand Oligomers of Antimicrobial Peptide Magainin 2 Contributes to Disruption of Phospholipid Membrane
| Content Provider | MDPI |
|---|---|
| Author | Kumashiro, Munehiro Tsuji, Ryoga Suenaga, Shoma Matsuo, Koichi |
| Copyright Year | 2022 |
| Description | The antimicrobial peptide magainin 2 (M2) interacts with and induces structural damage in bacterial cell membranes. Although extensive biophysical studies have revealed the interaction mechanism between M2 and membranes, the mechanism of membrane-mediated oligomerization of M2 is controversial. Here, we measured the synchrotron-radiation circular dichroism and linear dichroism (LD) spectra of M2 in dipalmitoyl-phosphatidylglycerol lipid membranes in lipid-to-peptide (L/P) molar ratios from 0–26 to characterize the conformation and orientation of M2 on the membrane. The results showed that M2 changed from random coil to α-helix structures via an intermediate state with increasing L/P ratio. Singular value decomposition analysis supported the presence of the intermediate state, and global fitting analysis revealed that M2 monomers with an α-helix structure assembled and transformed into M2 oligomers with a β-strand-rich structure in the intermediate state. In addition, LD spectra showed the presence of β-strand structures in the intermediate state, disclosing their orientations on the membrane surface. Furthermore, fluorescence spectroscopy showed that the formation of β-strand oligomers destabilized the membrane structure and induced the leakage of calcein molecules entrapped in the membrane. These results suggest that the formation of β-strand oligomers of M2 plays a crucial role in the disruption of the cell membrane. |
| Starting Page | 131 |
| e-ISSN | 20770375 |
| DOI | 10.3390/membranes12020131 |
| Journal | Membranes |
| Issue Number | 2 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-21 |
| Access Restriction | Open |
| Subject Keyword | Membranes Biophysics Amyloid Antimicrobial Peptide Β-strand Oligomers Fluorescence Anisotropy Linear Dichroism Magainin 2 Peptide-membrane Interaction Synchrotron-radiation Circular Dichroism |
| Content Type | Text |
| Resource Type | Article |
