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Antibacterial Activity of a Novel Oligosaccharide from Streptomyces californics against Erwinia carotovora subsp. Carotovora
| Content Provider | MDPI |
|---|---|
| Author | Al-Zubairy, Maysoon Abdulrahman Hussein, Khaled Alkhyat, Salwa H. Al-Mahdi, Abdullah Yahya Alghalibi, Saeed Munassar Al-Gheethi, Adel Ali Al-Shaibani, Muhanna Mohammed Enshasy, Hesham Ali El Sidik, Nik Marzuki |
| Copyright Year | 2022 |
| Description | The present study aims to characterize and predict models for antibacterial activity of a novel oligosaccharide from Streptomyces californics against Erwinia carotovora subsp. carotovora using an adaptive neuro-fuzzy inference system and an artificial neural network. The mathematical predication models were used to determine the optimal conditions to produce oligosaccharide and determine the relationship between the factors (pH, temperature, and time). The characteristics of the purified antibacterial agent were determined using ultraviolet spectroscopy (UV/Vis), infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy $(^{1}$H- and$ ^{13}$C-NMR), and mass spectrometry (MS). The best performances for the model were 39.45 and 35.16 recorded at epoch 1 for E. carotovora $Erw_{5}$ and E. carotovora EMCC 1687, respectively. The coefficient $(R^{2}$) of the training was more than 0.90. The highest antimicrobial production was recorded after 9 days at 25 °C and a pH of 6.2, at which more than 17 mm of the inhibition zone was obtained. The mass spectrum of antimicrobial agent (peak at R.T. = 3.433 of fraction 6) recorded two molecular ion peaks at m/z = 703.70 and m/z = 338.30, corresponding to molecular weights of 703.70 and 338.30 g/mol, respectively. The two molecular ion peaks matched well with the molecular formulas $C_{29}H_{53}NO_{18}$ and $C_{14}H_{26}O_{9}$, respectively, which were obtained from the elemental analysis result. A novel oligosaccharide from Streptomyces californics with potential activity against E. carotovora EMCC 1687 and E. carotovora $Erw_{5}$ was successfully isolated, purified, and characterized. |
| Starting Page | 2384 |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules27082384 |
| Journal | Molecules |
| Issue Number | 8 |
| Volume Number | 27 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-04-07 |
| Access Restriction | Open |
| Subject Keyword | Molecules Microbiology Antibacterial Activity Optimization Oligosaccharide Streptomyces Californics |
| Content Type | Text |
| Resource Type | Article |
