NDLI: Isolation and Characterization of Phages Active against Paenibacillus larvae Causing American Foulbrood in Honeybees in Poland

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Isolation and Characterization of Phages Active against Paenibacillus larvae Causing American Foulbrood in Honeybees in Poland

Content Provider	MDPI
Author	Ewa, Jończyk-Matysiak Kinga, Świtała-Jeleń Pawe, ł Migdał Martyna, Cieślik Norbert, Łodej Natalia, Bagińska Miros, ława Skupińska Pawe, ł Chorbiński Beata, Weber-Dąbrowska Andrzej, Górski Owczarek, Barbara Popiela, Ewa Kula, Dominika Neuberg, Joanna Hodyra-Stefaniak, Katarzyna Kaszowska, Marta Orwat, Filip Mucha, Anna Belter, Agnieszka Bubak, Barbara Fortuna, Wojciech Letkiewicz, Sławomir Roman, Adam
Copyright Year	2021
Description	The aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against Paenibacillus larvae, the causative agent of American Foulbrood—a highly infective and easily spreading disease occurring in honeybee larva, and subsequently the development of a preparation to prevent and treat this dangerous disease. From the tested material (over 2500 samples) 35 Paenibacillus spp. strains were obtained and used to search for phages. Five phages specific to Paenibacillus were isolated and characterized (ultrastructure, morphology, biological properties, storage stability, and genome sequence). The characteristics were performed to obtain knowledge of their lytic potential and compose the final phage cocktail with high antibacterial potential and intended use of future field application. Preliminary safety studies have also been carried out on healthy bees, which suggest that the phage preparation administered is harmless.
Starting Page	1217
e-ISSN	19994915
DOI	10.3390/v13071217
Journal	Viruses
Issue Number	7
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-06-23
Access Restriction	Open
Subject Keyword	Viruses Integrative and Complementary Medicine American Foulbrood Honeybee Bacteriophages Phage Isolation Paenibacillus Larvae
Content Type	Text
Resource Type	Article

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