Loading...
Please wait, while we are loading the content...
Similar Documents
Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity
| Content Provider | MDPI |
|---|---|
| Author | Roberto, T. Abdala-Díaz Claudia, Pérez Carla, Durán Figueroa, Fabian A. Casas-Arrojo, Virginia Nesic, Aleksandra Tapia, Cecilia Valdes, Oscar Parra, Carolina Bravo-Arrepol, Gastón Soto, Luis Becerra, José Cabrera-Barjas, Gustavo |
| Copyright Year | 2022 |
| Description | Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the $ABTS^{•+}$ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg $mL^{−1}$ concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg $mL^{−1}$. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource. |
| Starting Page | 458 |
| e-ISSN | 16603397 |
| DOI | 10.3390/md20070458 |
| Journal | Marine drugs |
| Issue Number | 7 |
| Volume Number | 20 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-07-15 |
| Access Restriction | Open |
| Subject Keyword | Marine drugs Marine Drugs Integrative and Complementary Medicine Codium Bernabei Sulfated Galactans Polysaccharides Anticoagulant Activity Antioxidant Capacity Cytotoxic Effect |
| Content Type | Text |
| Resource Type | Article |
