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Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
| Content Provider | MDPI |
|---|---|
| Author | Thomas, E. Mürdter Becker, Lucas Janssen, Nicole Layland, Shannon L. Nies, Anne T. Schenke-Layland, Katja Marzi, Julia |
| Copyright Year | 2021 |
| Description | Hurdles for effective tumor therapy are delayed detection and limited effectiveness of systemic drug therapies by patient-specific multidrug resistance. Non-invasive bioimaging tools such as fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved over the last decade, providing the potential to be translated into clinics for early-stage disease detection, in vitro drug screening, and drug efficacy studies in personalized medicine. Accessing tissue- and cell-specific spectral signatures, Raman microspectroscopy has emerged as a diagnostic tool to identify precancerous lesions, cancer stages, or cell malignancy. In vivo Raman measurements have been enabled by recent technological advances in Raman endoscopy and signal-enhancing setups such as coherent anti-stokes Raman spectroscopy or surface-enhanced Raman spectroscopy. FLIM enables in situ investigations of metabolic processes such as glycolysis, oxidative stress, or mitochondrial activity by using the autofluorescence of co-enzymes NADH and FAD, which are associated with intrinsic proteins as a direct measure of tumor metabolism, cell death stages and drug efficacy. The combination of non-invasive and molecular-sensitive in situ techniques and advanced 3D tumor models such as patient-derived organoids or microtumors allows the recapitulation of tumor physiology and metabolism in vitro and facilitates the screening for patient-individualized drug treatment options. |
| Starting Page | 5682 |
| e-ISSN | 20726694 |
| DOI | 10.3390/cancers13225682 |
| Journal | Cancers |
| Issue Number | 22 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-11-13 |
| Access Restriction | Open |
| Subject Keyword | Cancers Oncology Raman Microspectroscopy Fluorescence Lifetime Imaging Microscopy Tissue Diagnostics Tumor Metabolism in Situ Imaging 3d in Vitro Models |
| Content Type | Text |
| Resource Type | Article |
