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Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid
| Content Provider | MDPI |
|---|---|
| Author | Qi, Beibei Wu, Chao Liang, Huiling Cui, Kehui Fahad, Shah Wang, Manlian Liu, Baoyu Nie, Lixiao Huang, Jianliang Tang, Hui |
| Copyright Year | 2021 |
| Description | Liquid-liquid extraction and solid phase extraction followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing the performance of the HPLC-UV method, including composition of the mobile phase for gradient elution, column temperature, flow rate, and detection wavelength, were optimized. This method can simultaneously determine 11 phytohormones, including nine cytokinins, indole-3-acetic acid, and abscisic acid. The limit of detection of this method is 0.22 to 1.1 µg $L^{−1}$, and the coefficient factors of linear regression are >0.998. The recoveries of the target phytohormones ranged between 62.1~109.4%, and the relative standard deviations were <10%. This method is suitable for determining phytohormones, especially cytokinins, in young panicles, roots, and xylem sap of rice plants. |
| Starting Page | 6998 |
| e-ISSN | 20711050 |
| DOI | 10.3390/su13136998 |
| Journal | Sustainability |
| Issue Number | 13 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-06-22 |
| Access Restriction | Open |
| Subject Keyword | Sustainability Hplc-uv Cytokinin Indole-3-acetic Acid Abscisic Acid |
| Content Type | Text |
| Resource Type | Article |
