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Experimental Measurement of Sharp THz Absorption Signatures from Nucleic Acid Solutions in Nanofluidic Channels
| Content Provider | MDPI |
|---|---|
| Author | Brown, Elliott R. Mendoza, Edgar A. |
| Copyright Year | 2021 |
| Description | We report on the room-temperature experimental measurement of THz absorption signatures in aqueous, double-stranded nucleic acid solutions confined to the submicron silica channels on fused quartz substrates using THz frequency-domain (photomixing) spectroscopy. Three sharp (i.e., strong and narrow) signatures, ~10–20 GHz FWHM, are observed in the shortest base pair sample—small interfering, double-stranded (ds) RNA—in the range of 800 GHz to 1.1 THz. Three similar signatures are also observed in a 50-bp dsDNA ladder sample. For a 1-kbp dsDNA ladder sample, the three are still evident, but are broadened and weakened. For a 48.5-kbp sample (λ-DNA), no prominent signatures are observed, but rather a quasi-sinusoidal transmittance spectrum consistent with a substrate etalon effect. The division between sharp signatures and no signatures is consistent with the molecular length being shorter or longer than the persistence length. |
| Starting Page | 11827 |
| e-ISSN | 20763417 |
| DOI | 10.3390/app112411827 |
| Journal | Applied Sciences |
| Issue Number | 24 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-13 |
| Access Restriction | Open |
| Subject Keyword | Applied Sciences Urology and Nephrology Thz Spectroscopy Thz Absorption Signatures Nanofluidic Chips Nanochannels Double-stranded Rna Double-stranded Dna Molecular Persistence Length |
| Content Type | Text |
| Resource Type | Article |
