NDLI: Papain Ameliorates Lipid Accumulation and Inflammation in High-Fat Diet-Induced Obesity Mice and 3T3-L1 Adipocytes via AMPK Activation

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Papain Ameliorates Lipid Accumulation and Inflammation in High-Fat Diet-Induced Obesity Mice and 3T3-L1 Adipocytes via AMPK Activation

Content Provider	MDPI
Author	Kang, Yun-Mi Kang, Hyun-Ae Cominguez, Divina C. Kim, Su-Hyun An, Hyo-Jin
Copyright Year	2021
Description	Papain is a proteolytic enzyme present in the leaves, fruits, roots, and latex of the Carica papaya (papaya) plant. Although it exhibits a wide range of activities, there are no reports on the anti-obesity effects of papain. This study examined the anti-obesity effect and obesity-involved anti-inflammatory mechanism of papain in in vivo and in vitro models using high-fat diet (HFD)-induced obese mice and 3T3-L1 preadipocytes. Oral administration of papain reduced HFD-induced weight of the body, liver, and adipose tissues of mice. Papain also reduced hepatic lipid accumulation and adipocyte size. Moreover, serum total cholesterol and triglyceride levels were markedly reduced in papain-treated mice. In addition, papain inhibited the differentiation of preadipocytes and oil accumulation in 3T3-L1 preadipocytes and rat primary preadipocytes. Mechanistically, papain significantly downregulated the protein levels of key adipogenesis regulators and reversed the expression of pro-inflammatory cytokines and adipokines in HFD-induced obese mice and 3T3-L1 preadipocytes. Papain also markedly enhanced activation of the AMP-activated protein kinase pathway in both models. Collectively, these results suggest that papain exerts anti-obesity effects in HFD-induced mice and 3T3-L1 preadipocytes by regulating levels of adipogenic factors involved in lipid metabolism and inflammation; thus, it could be useful in the prevention and treatment of obesity.
Starting Page	9885
e-ISSN	14220067
DOI	10.3390/ijms22189885
Journal	International Journal of Molecular Sciences
Issue Number	18
Volume Number	22
Language	English
Publisher	MDPI
Publisher Date	2021-09-14
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Papain Carica Papaya Obesity Inflammation High-fat Diet 3t3-l1 Preadipocytes Adipogenesis Amp-activated Protein Kinase
Content Type	Text
Resource Type	Article

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