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Investigation of Total Phenolic Content and Antioxidant Activities of Spruce Bark Extracts Isolated by Deep Eutectic Solvents
| Content Provider | MDPI |
|---|---|
| Author | Jablonsky, Michal Majova, Veronika Strizincova, Petra Sima, Jozef Jablonsky, Jozef |
| Copyright Year | 2020 |
| Description | Extracts from spruce bark obtained using different deep eutectic solvents were screened for their total phenolic content (TPC) and antioxidant activities. Water containing choline chloride-based deep eutectic solvents (DESs) with lactic acid and 1,3-propanediol, 1,3-butanediol, 1,4-butanediol, and 1,5-pentanediol, with different molar ratios, were used as extractants. Basic characteristics of the DESs (density, viscosity, conductivity, and refractive index) were determined. All the DESs used behave as Newtonian liquids. The extractions were performed for 2 h at 60 °C under continuous stirring. TPC was determined spectrophotometrically, using the Folin-Ciocalteu reagent, and expressed as gallic acid equivalent (GAE). The antioxidant activity was determined spectrophotometrically by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The TPC varied from 233.6 to 596.2 mg GAE/100 g dry bark; radical scavenging activity (RSA) ranged between 81.4% and 95%. This study demonstrated that deep eutectic solvents are suitable solvents for extracting phenolic compounds from spruce bark. |
| Starting Page | 402 |
| e-ISSN | 20734352 |
| DOI | 10.3390/cryst10050402 |
| Journal | Crystals |
| Issue Number | 5 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-05-16 |
| Access Restriction | Open |
| Subject Keyword | Crystals Paper and Wood Deep Eutectic Solvents Phenolic Compounds Antioxidant Activity Spruce Bark Extraction |
| Content Type | Text |
| Resource Type | Article |