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The Effect of Different Substrates on the Morphological Features and Polyols Production of Endomyces magnusii Yeast during Long-Lasting Cultivation
| Content Provider | MDPI |
|---|---|
| Author | Kokoreva, Anastasia S. Isakova, Elena P. Tereshina, Vera M. Klein, Olga I. Gessler, Natalya N. Deryabina, Yulia I. |
| Copyright Year | 2022 |
| Description | The study on the influence of different glucose concentrations (2%, 0.5%, and 0.2%) and glycerol (1%) on the morphological and physiological features, as well as the composition of soluble carbohydrates, was performed using Endomyces magnusii yeast. Two-factor analysis of variance with repetitions to process the data of the cell size changes showed that the substrate type affected cell size the most. The cells with 2% glucose were 30–35% larger than those growing on glycerol. The decrease in the initial glucose concentration up to 0.5–0.2% slightly changed the cell length. However, even in the logarithmic growth phase pseudo-mycelium of two to four cells appeared in the cultures when using low glucose, unlike those using glycerol. Throughout the whole experiment, more than 90% of the populations remained viable on all of the substrates tested. The ability for colony formation decreased during aging. Nevertheless, at the three-week stage, upon substrate restriction (0.2% glucose), it was twice higher than those under the other conditions. The respiration rate also decreased and exceeded not more than 10% of that in the logarithmic phase. By the end of the experiment, the cyanide-sensitive respiration share decreased up to 40% for all types of substrates. The study of soluble cytosol carbohydrates showed that the cultures using 2% glucose and 1% glycerol contained mainly arabitol and mannitol, while at low glucose concentrations they were substituted for inositol. The formation of inositol is supposed to be related to pseudo-mycelium formation. The role of calorie restriction in the regulation of carbohydrate synthesis and the composition in the yeast and its biotechnological application is under consideration. |
| Starting Page | 1709 |
| e-ISSN | 20762607 |
| DOI | 10.3390/microorganisms10091709 |
| Journal | Microorganisms |
| Issue Number | 9 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-08-25 |
| Access Restriction | Open |
| Subject Keyword | Microorganisms Microbiology Endomyces Magnusii Mitochondria Yeast Long-lasting Cultivation Cytosol Carbohydrates |
| Content Type | Text |
| Resource Type | Article |
