NDLI: Thymoquinone-Loaded Polymeric Films and Hydrogels for Bacterial Disinfection and Wound Healing

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Thymoquinone-Loaded Polymeric Films and Hydrogels for Bacterial Disinfection and Wound Healing

Content Provider	MDPI
Author	Haq, Anika Kumar, Suneel Mao, Yong Berthiaume, Francois Michniak-Kohn, Bozena
Copyright Year	2020
Description	The purpose of this study was to synthesize and characterize novel biocompatible topical polymeric film and hydrogel systems that have the potential to deliver the antibacterial agent thymoquinone (TQ) directly to the skin target site to manage the local wound infection and thereby wound healing. The polyvinyl pyrrolidone (PVP) matrix-type films containing TQ were prepared by the solvent casting method. In vitro skin permeation studies on human cadaver skin produced a mean flux of 2.3 µg TQ/cm2/h. Human keratinocyte monolayers subjected to a scratch wound (an in vitro wound healing assay) showed 85% wound closure at day 6 in the TQ group (100 ng/mL TQ) as compared to 50% in the vehicle control group (p = 0.0001). In a zone-of-inhibition (ZOI) assay, TQ-containing films and hydrogels completely wiped out Staphylococcus aureus in 10 cm diameter Tryptic Soy Agar plates while 500 µg/mL gentamicin containing filters gave 10 mm of ZOI. In an ex vivo model, TQ-containing films eradicated bacterial colonization on human cadaver skin. Furthermore, in a full-thickness wound infection model in mice, TQ-containing films showed significant activity in controlling Staphylococcus aureus infection, thereby disinfecting the skin wound. In summary, TQ-containing PVP films and hydrogels developed in this study have the potential to treat and manage wound infections.
Starting Page	386
e-ISSN	22279059
DOI	10.3390/biomedicines8100386
Journal	Biomedicines
Issue Number	10
Volume Number	8
Language	English
Publisher	MDPI
Publisher Date	2020-09-28
Access Restriction	Open
Subject Keyword	Biomedicines Cell Tissue Engineering Thymoquinone Polymeric Film and Hydrogel Topical/transdermal Drug Delivery Wound Disinfection Staphylococcus Aureus Bacterial Skin Infections
Content Type	Text
Resource Type	Article

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