NDLI: Implementation of a Rapid RT-LAMP Saliva-Based SARS-CoV-2 Testing Program in the Workplace

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Implementation of a Rapid RT-LAMP Saliva-Based SARS-CoV-2 Testing Program in the Workplace

Content Provider	MDPI
Author	Cook, Bradley W. M. Kobasa, Kaitlyn Tamayo, Marielou Theriault, Natasha Pappas, Diane Gordon Theriault, Steven S.
Copyright Year	2022
Description	Rising SARS-CoV-2 cases, testing delays, and the risk of pre-symptomatic and asymptomatic transmission provided the impetus for an in-house rapid testing program. Employees and their household contacts were encouraged to self-collect saliva samples that were pooled for routine testing using an established colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. In brief, individual or a maximum of four saliva samples were pooled and heat-inactivated to render microorganisms, especially SARS-CoV-2, non-infectious prior to being added to RT-LAMP assay tubes containing either the human sample control gene, RNase P, or a region of the SARS-CoV-2 gene, ORF1ab. During the second wave of SARS-CoV-2 infections in November 2020, two samples from an employee and a member of their household tested positive via RT-LAMP within two days of each other. A delayed clinical qRT-PCR test confirmation of both individuals 5 days later underscored the power of routine rapid testing with within-the-hour turnaround times. Workplace rapid testing programs using RT-LAMP are flexible in their design, have a reduced cost compared to qRT-PCR, may involve non-invasive self-saliva collection for increased safety for the testing personnel, and can be performed with minimal training.
Starting Page	474
e-ISSN	20754418
DOI	10.3390/diagnostics12020474
Journal	Diagnostics
Issue Number	2
Volume Number	12
Language	English
Publisher	MDPI
Publisher Date	2022-02-12
Access Restriction	Open
Subject Keyword	Diagnostics Microbiology Sars-cov-2 Covid-19 Rapid Molecular Testing Rt-lamp Occupational Health and Safety Group Testing Self-administration Test
Content Type	Text
Resource Type	Article

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