NDLI: The Role of MAPK3/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium

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The Role of MAPK3/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium

Content Provider	MDPI
Author	Radek, Procházka Alexandra, Bartková Lucie, Němcová Matej, Murín Kate, řina Marcollová Veronika, Kinterová Jozef, Laurinčík Gad, Ahmed Lucas-Hahn, Andrea
Copyright Year	2021
Description	The developmental potential of porcine oocytes cultured in vitro was remarkably enhanced in a medium containing FGF2, LIF and IGF1 (FLI) when compared to a medium supplemented with gonadotropins and EGF (control). We analyzed the molecular background of the enhanced oocyte quality by comparing the time course of MAPK3/1 and AKT activation, and the expression of genes controlled by these kinases in cumulus-oocyte complexes (COCs) cultured in FLI and the control medium. The pattern of MAPK3/1 activation in COCs was very similar in both media, except for a robust increase in MAPK3/1 phosphorylation during the first hour of culture in the FLI medium. The COCs cultured in the FLI medium exhibited significantly higher activity of AKT than in the control medium from the beginning up to 16 h of culture; afterwards a deregulation of AKT activity occurred in the FLI medium, which was not observed in the control medium. The expression of cumulus cell genes controlled by both kinases was also modulated in the FLI medium, and in particular the genes related to cumulus-expansion, signaling, apoptosis, antioxidants, cell-to-cell communication, proliferation, and translation were significantly overexpressed. Collectively, these data indicate that both MAPK3/1 and AKT are implicated in the enhanced quality of oocytes cultured in FLI medium.
Starting Page	11148
e-ISSN	14220067
DOI	10.3390/ijms222011148
Journal	International Journal of Molecular Sciences
Issue Number	20
Volume Number	22
Language	English
Publisher	MDPI
Publisher Date	2021-10-15
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Reproductive Biology Fgf2 Lif Igf1 Oocyte Maturation Oocyte Competence Map Kinase 3/1 Akt Kinase Gene Expression
Content Type	Text
Resource Type	Article

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