NDLI: Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer

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Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer

Content Provider	MDPI
Author	Karol, ína Trachtová Wiebringhaus, Robert Pecoraro, Matteo Neubauer, Heidi A. Trimmel, Bettina Wieselberg, Maritta Pencik, Jan Egger, Gerda Krall, Christoph Moriggl, Richard Mann, Matthias Hantusch, Brigitte Kenner, Lukas
Copyright Year	2021
Description	We aimed to identify novel markers for aggressive prostate cancer in a STAT3-low proteomics-derived dataset of mitochondrial proteins by immunohistochemical analysis and correlation with transcriptomic data and biochemical recurrence in a STAT3 independent PCa cohort. Formalin-fixed paraffin-embedded tissue (FFPE) sample selection for proteomic analysis and tissue-microarray (TMA) generation was conducted from a cohort of PCa patients. Retrospective data analysis was performed with the same cohort. 153 proteins differentially expressed between STAT3-low and STAT3-high samples were identified. Out of these, 46 proteins were associated with mitochondrial processes including oxidative phosphorylation (OXPHOS), and 45 proteins were upregulated, including NDUFS1/ATP5O. In a STAT3 independent PCa cohort, high expression of NDUFS1/ATP5O was confirmed by immunocytochemistry (IHC) and was significantly associated with earlier biochemical recurrence (BCR). mRNA expression levels for these two genes were significantly higher in intra-epithelial neoplasia and in PCa compared to benign prostate glands. NDUFS1/ATP5O levels are increased both at the mRNA and protein level in aggressive PCa. Our results provide evidence that NDUFS1/ATP5O could be used to identify high-risk PCa patients.
Starting Page	6036
e-ISSN	20726694
DOI	10.3390/cancers13236036
Journal	Cancers
Issue Number	23
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-11-30
Access Restriction	Open
Subject Keyword	Cancers Urology and Nephrology Prostate Cancer Ffpe-proteomics Oxphos Ndufs1 Atp5o Stat3 Transcriptomics
Content Type	Text
Resource Type	Article

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