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Sunset Yellow Dye Induces Amorphous Aggregation in β-Lactoglobulin at Acidic pH: A Multi-Techniques Approach
| Content Provider | MDPI |
|---|---|
| Author | Khan, Javed Masood Malik, Ajamaluddin Husain, Fohad Mabood Hakeem, Mohammed J. Alhomida, Abdullah S. |
| Copyright Year | 2022 |
| Description | Protein aggregation is of two types: (i) amorphous and (ii) amyloid fibril. Several extrinsic factors (temperature, pH, and small ligands) stimulate protein aggregation in vitro. In this study, we have examined the role of sunset yellow (SY) on the β-lactoglobulin (BLG) aggregation at pH 2.0. We have used spectroscopic (turbidity, Rayleigh light scattering (RLS), far-UV CD) and microscopic (transmission electron microscopy [TEM]) techniques to describe the effects of SY on BLG aggregation. Our results showed that BLG aggregation is dependent on SY concentrations. Very low concentrations (0.0–0.07 mM) of SY were unable to induce aggregation, while SY in the concentrations range of 0.1–5.0 mM induces aggregation in BLG. The kinetics of SY-stimulated aggregation is very fast and monomeric form of BLG directly converted into polymeric aggregates. The kinetics results also showed SY-induced BLG aggregation disappeared in the presence of NaCl. The far-UV CD and TEM results indicated the amorphous nature of SY-induced BLG aggregates. We believe that our results clearly suggest that SY dye effectively stimulates BLG aggregation. |
| Starting Page | 395 |
| e-ISSN | 20734360 |
| DOI | 10.3390/polym14030395 |
| Journal | Polymers |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-20 |
| Access Restriction | Open |
| Subject Keyword | Polymers Analytical Chemistry Β-lactoglobulin Sunset Yellow Amorphous Aggregation Acidic Ph Whey Protein |
| Content Type | Text |
| Resource Type | Article |
