NDLI: Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells

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Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells

Content Provider	MDPI
Author	Marta, Cadenas-Martin Adrian, Moratilla Jorge, Fernández-Delgado Francisco, Arnalich-Montiel Miguel, Maria P. De
Copyright Year	2021
Description	Corneal disease affects 12.5 million individuals worldwide, with 2 million new cases each year. The standard treatment consists of a corneal transplantation from a human donor; however, the worldwide demand significantly exceeds the available supply. Lamellar endothelial keratoplasty, the replacement of only the endothelial layer of the cornea, can partially solve the problem. Progressive efforts have s쳮ded in expanding hCECs; however, the ability to expand hCECs is still limited, and new sources of CECs are being sought. Crucial advances have been achieved by the directed differentiation of embryonic or induced pluripotent stem cells, but these cells have disadvantages, such as the use of oncogenes, and are still difficult to establish. We aimed to transfer such knowledge to obtain hCECs from adipose tissue-derived adult mesenchymal stem cells (ADSC) by modifying four previously published procedures. We present several protocols capable of the directed differentiation of human ADSCs to hCECs. In our hands, the protocol by Ali et al. was the best adapted to such differentiation in terms of efficiency, time, and financial cost; however, the protocol by Wagoner et al. was the best for CEC marker expression. Our results broaden the type of cells of autologous extraocular origin that could be employed in the clinical setting for corneal endothelial deficiency.
Starting Page	11982
e-ISSN	14220067
DOI	10.3390/ijms222111982
Journal	International Journal of Molecular Sciences
Issue Number	21
Volume Number	22
Language	English
Publisher	MDPI
Publisher Date	2021-11-05
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Cell Tissue Engineering Cell Reprogramming Differentiation Adipose Tissue-derived Mesenchymal Stem Cells Cornea Corneal Endothelium
Content Type	Text
Resource Type	Article

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