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Designing Functionally Versatile, Highly Immunogenic Peptide-Based Multiepitopic Vaccines against Foot-and-Mouth Disease Virus
| Content Provider | MDPI |
|---|---|
| Author | Rodrigo, Cañas-Arranz Patricia, De León Miguel, Rodríguez-Pulido Defaus, Sira Forner, Mar Bustos, María J. Blanco, Esther Sobrino, Francisco Andreu, David |
| Copyright Year | 2020 |
| Description | A broadly protective and biosafe vaccine against foot-and-mouth disease virus (FMDV) remains an unmet need in the animal health sector. We have previously reported solid protection against serotype O FMDV afforded by dendrimeric peptide structures harboring virus-specific B- and T-cell epitopes, and also shown such type of multivalent presentations to be advantageous over simple B-T-epitope linear juxtaposition. Chemically, our vaccine platforms are modular constructions readily made from specified B- and T-cell epitope precursor peptides that are conjugated in solution. With the aim of developing an improved version of our formulations to be used for on-demand vaccine applications, we evaluate in this study a novel design for epitope presentation to the immune system based on a multiple antigen peptide (MAP) containing six immunologically relevant motifs arranged in dendrimeric fashion (named B2T-TB2). Interestingly, two B2T units fused tail-to-tail into a single homodimer platform elicited higher B- and T-cell specific responses than former candidates, with immunization scores remaining stable even after 4 months. Moreover, this macromolecular assembly shows consistent immune response in swine, the natural FMDV host, at reduced dose. Thus, our versatile, immunogenic prototype can find application in the development of peptide-based vaccine candidates for various therapeutic uses using safer and more efficacious vaccination regimens. |
| Starting Page | 406 |
| e-ISSN | 2076393X |
| DOI | 10.3390/vaccines8030406 |
| Journal | Vaccines |
| Issue Number | 3 |
| Volume Number | 8 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-07-22 |
| Access Restriction | Open |
| Subject Keyword | Vaccines Immunology Peptide-based Vaccines Foot-and-mouth Disease Virus Multivalency Click Chemistry Swine Host |
| Content Type | Text |
| Resource Type | Article |
