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Genomic Analysis and Secondary Metabolites Production of the Endophytic Bacillus velezensis Bvel1: A Biocontrol Agent against Botrytis cinerea Causing Bunch Rot in Post-Harvest Table Grapes
| Content Provider | MDPI |
|---|---|
| Author | Nifakos, Kallimachos Tsalgatidou, Polina C. Thomloudi, Eirini-Evangelia Skagia, Aggeliki Kotopoulis, Dimitrios Baira, Eirini Delis, Costas Papadimitriou, Konstantinos Markellou, Emilia Venieraki, Anastasia Katinakis, Panagiotis |
| Copyright Year | 2021 |
| Description | Botrytis bunch rot caused by Botrytis cinerea is one of the most economically significant post-harvest diseases of grapes. In the present study, we showed that the bacterial strain Bvel1 is phylogenetically affiliated to Bacillus velezensis species. The strain Bvel1 and its secreted metabolites exerted an antifungal activity, under in vitro conditions, against B. cinerea. UHPLC–HRMS chemical analysis revealed that iturin A2, surfactin-C13 and -C15, oxydifficidin, bacillibactin, L-dihydroanticapsin, and azelaic acid were among the metabolites secreted by Bvel1. Treatment of wounded grape berries with Bacillus sp. Bvel1 cell culture was effective for controlling grey mold ingress and expansion in vivo. The effectiveness of this biological control agent was a function of the cell culture concentration of the antagonist applied, while preventive treatment proved to be more effective compared to curative. The strain Bvel1 exhibited an adequate colonization efficiency in wounded grapes. The whole-genome phylogeny, combined with ANI and dDDH analyses, provided compelling evidence that the strain Bvel1 should be taxonomically classified as Bacillus velezensis. Genome mining approaches showed that the strain Bvel1 harbors 13 antimicrobial biosynthetic gene clusters, including iturin A, fengycin, surfactin, bacilysin, difficidin, bacillaene, and bacillibactin. The results provide new insights into the understanding of the endophytic Bacillus velezensis Bvel1 biocontrol mechanism against post-harvest fungal pathogens, including bunch rot disease in grape berries. |
| Starting Page | 1716 |
| e-ISSN | 22237747 |
| DOI | 10.3390/plants10081716 |
| Journal | Plants |
| Issue Number | 8 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-20 |
| Access Restriction | Open |
| Subject Keyword | Plants Microbiology Endophytic Bacteria Metabolites Iturin A Surfactin Genome Sequence Colonization Biological Control Induced Systemic Resistance |
| Content Type | Text |
| Resource Type | Article |
