Loading...
Please wait, while we are loading the content...
Similar Documents
Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice
| Content Provider | MDPI |
|---|---|
| Author | Vlatka, Paštar Mirela, Lozić Natalija, Filipović Katarina, Vukojević Kelam, Nela Bernard, Branka Katsuyama, Yu |
| Copyright Year | 2021 |
| Description | Disabled-1 (Dab1) protein is an intracellular adaptor of reelin signaling required for prenatal neuronal migration, as well as postnatal neurotransmission, memory formation and synaptic plasticity. Yotari, an autosomal recessive mutant of the mouse Dab1 gene is recognizable by its premature death, unstable gait and tremor. Previous findings are mostly based on neuronal abnormalities caused by Dab1 deficiency, but the role of the reelin signaling pathway in nonneuronal tissues and organs has not been studied until recently. Hepatocytes, the most abundant cells in the liver, communicate via gap junctions (GJ) are composed of connexins. Cell communication disruption in yotari mice was examined by analyzing the expression of connexins (Cxs): Cx26, Cx32, Cx37, Cx40, Cx43 and Cx45 during liver development at 13.5 and 15.5 gestation days (E13.5 and E15.5). Analyses were performed using immunohistochemistry and fluorescent microscopy, followed by quantification of area percentage covered by positive signal. Data are expressed as a mean ± SD and analyzed by one-way ANOVA. All Cxs examined displayed a significant decrease in yotari compared to wild type (wt) individuals at E13.5. Looking at E15.5 we have similar results with exception of Cx37 showing negligible expression in wt. Channels formation triggered by pathological stimuli, as well as propensity to apoptosis, was studied by measuring the expression of Pannexin1 (Panx1) and Apoptosis-inducing factor (AIF) through developmental stages mentioned above. An increase in Panx1 expression of E15.5 yotari mice, as well as a strong jump of AIF in both phases suggesting that yotari mice are more prone to apoptosis. Our results emphasize the importance of gap junction intercellular communication (GJIC) during liver development and their possible involvement in liver pathology and diagnostics where they can serve as potential biomarkers and drug targets. |
| Starting Page | 10712 |
| e-ISSN | 14220067 |
| DOI | 10.3390/ijms221910712 |
| Journal | International Journal of Molecular Sciences |
| Issue Number | 19 |
| Volume Number | 22 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-10-02 |
| Access Restriction | Open |
| Subject Keyword | International Journal of Molecular Sciences Cell Tissue Engineering Gap Junction Liver Development Cx26 Cx32 Cx37 Cx40 Cx43 Cx45 Panx1 Aif Yotari |
| Content Type | Text |
| Resource Type | Article |
