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Fluorescent AgNCs Formed on Bifunctional DNA Template for Potassium Ion Detection
| Content Provider | MDPI |
|---|---|
| Author | Angelika, Świtalska Filipczuk, Patrycja Kosman, Joanna Nowaczyk, Grzegorz Dembska, Anna |
| Copyright Year | 2021 |
| Description | In this study, we examined properties of silver nanoclusters, which are AgNCs stabilized by DNA oligonucleotide scaffold containing G-quadruplex-forming sequences: human telomeric (Tel22) or thrombin-binding aptamer (TBA). Thus, we obtained two fluorescent probes abbreviated as Tel22C12-AgNCs and TBAC12-AgNCs, which were characterized using absorption, circular dichroism and fluorescence spectroscopy. Both probes emit green and red fluorescence. The presence of silver nanoclusters did not destabilize the formed G-quadruplexes. The structural changes of probes upon binding $K^{+}$ or $Na^{+}$ ions cause quenching in their red emission. Green emission was slightly quenched only in the case of Tel22C12-AgNCs; on the contrary, for TBAC12-AgNC’s green emission, we observed an increasing fluorescence signal. Moreover, the Tel22C12-AgNCs system shows not only a higher binding preference for $K^{+}$ over $Na^{+}$, but it was able to monitor small changes in $K^{+}$ concentrations in the buffer mimicking extracellular conditions (high content of $Na^{+}$ ions). These results suggest that Tel22C12-AgNCs exhibit the potential to monitor transmembrane potassium transport. |
| Starting Page | 349 |
| e-ISSN | 22279040 |
| DOI | 10.3390/chemosensors9120349 |
| Journal | Chemosensors |
| Issue Number | 12 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-09 |
| Access Restriction | Open |
| Subject Keyword | Chemosensors Silver Nanoclusters G-quadruplex Fluorescent Probe Potassium Sensing |
| Content Type | Text |
| Resource Type | Article |
