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Mapping and Analysis of Swi5 and Sfr1 Phosphorylation Sites
| Content Provider | MDPI |
|---|---|
| Author | Sevcovicova, Andrea Plava, Jana Gazdarica, Matej Szabova, Eva Huraiova, Barbora Gaplovska-Kysela, Katarina Cipakova, Ingrid Cipak, Lubos Gregan, Juraj |
| Copyright Year | 2021 |
| Description | The evolutionarily conserved Swi5-Sfr1 complex plays an important role in homologous recombination, a process crucial for the maintenance of genomic integrity. Here, we purified Schizosaccharomyces pombe Swi5-Sfr1 complex from meiotic cells and analyzed it by mass spectrometry. Our analysis revealed new phosphorylation sites on Swi5 and Sfr1. We found that mutations that prevent phosphorylation of Swi5 and Sfr1 do not impair their function but swi5 and sfr1 mutants encoding phosphomimetic aspartate at the identified phosphorylation sites are only partially functional. We conclude that during meiosis, Swi5 associates with Sfr1 and both Swi5 and Sfr1 proteins are phosphorylated. However, the functional relevance of Swi5 and Sfr1 phosphorylation remains to be determined. |
| Starting Page | 1014 |
| e-ISSN | 20734425 |
| DOI | 10.3390/genes12071014 |
| Journal | Genes |
| Issue Number | 7 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-06-30 |
| Access Restriction | Open |
| Subject Keyword | Genes Genetics Swi5 Sfr1 Phosphorylation Dna Repair Recombination Meiosis Schizosaccharomyces Pombe |
| Content Type | Text |
| Resource Type | Article |