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Structure and Conformation Study of the O-Antigen from the Lipopolysaccharide of Cupriavidus Metallidurans CH34
| Content Provider | MDPI |
|---|---|
| Author | Notaro, Anna Vanacore, Adele Molinaro, Antonio Speciale, Immacolata Castro, Cristina De |
| Copyright Year | 2022 |
| Description | Cupriavidus metallidurans is a Gram-negative bacterium that has attracted the attention of the scientific community since its discovery back in 1976. It was initially studied as a model organism for bioremediation processes due to its ability to survive in heavy metal-rich environments. However, in recent years, there is evidence that this bacterium can be a potential pathogen for humans. How C. metallidurans can survive in such different environments is unknown and prompted the following work. Its great adaptability could be explained by the structural and conformational studies of the O-antigen portion of the lipopolysaccharide, the main constituent of the outer membrane of Gram-negative bacteria, which is the one in direct contact with the external environment. Therefore, a combination of chemical and spectroscopic analyses was used to define the O-antigen structure, disclosing that it is a polysaccharide constituted of a linear tetrasaccharide repeating unit that does not resemble other structures already reported for bacteria: [4)-α-d-GalNAc-(1→3)-α-d-Qui2NAc4NHBA-(1→3)-α-l-Rha-(1→3)-α-l-Rha-(1→]. Interestingly, the molecular dynamics studies revealed that the three-dimensional structure of the O-antigen is highly flexible: it might adopt three different right-handed helix conformations described by a two, three, or four-fold symmetry. This conformational behavior could represent the reason behind the survival of C. metallidurans in different environments. |
| Ending Page | 199 |
| Page Count | 12 |
| Starting Page | 188 |
| e-ISSN | 26734176 |
| DOI | 10.3390/polysaccharides3010009 |
| Journal | Polysaccharides |
| Issue Number | 1 |
| Volume Number | 3 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-20 |
| Access Restriction | Open |
| Subject Keyword | Polysaccharides Microbiology Cupriavidus Metallidurans Ch34 Dynamic Simulation Nmr Spectroscopy O-antigen Structure |
| Content Type | Text |
| Resource Type | Article |
