NDLI: Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

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Immunogenicity of Recombinant-Deficient Lactobacillus casei with Complementary Plasmid Expressing Alanine Racemase Gene and Core Neutralizing Epitope Antigen against Porcine Epidemic Diarrhea Virus

Content Provider	MDPI
Author	Li, Fengsai Wang, Xiaona Fan, Xiaolong Sui, Ling Zhang, Hailin Li, Yue Zhou, Han Wang, Li Qiao, Xinyuan Tang, Lijie Li, Yijing
Copyright Year	2021
Description	Porcine epidemic diarrhea (PED), which is caused by the porcine epidemic diarrhea virus (PEDV), has occurred worldwide and poses a serious threat to the pig industry. Intestine is the main function site of PEDV; therefore, it is important to develop an oral mucosal immunity vaccine against this virus infection. Most traditional plasmid delivery vectors use antibiotic genes as a selective marker, easily leading to antibiotic accumulation and gene contamination. In this study, to explore whether the alanine racemase gene (Alr) could be used as a screening marker and develop an efficient oral vaccine against PEDV infection, a recombinant strain was constructed using Lactobacillus casei with Alr deletion (L. casei ΔAlr W56) to deliver the Alr gene and a core-neutralizing epitope (COE) antigen. This recombinant bacterium efficiently induced secretory immunoglobulin A (SIgA)-based mucosal and immunoglobulin G (IgG)-based humoral immune responses via oral vaccination in mice. Compared to the other strains, the recombinant bacteria were able to grow without the addition of D-alanine, revealing that Alr in the plasmid could function normally in defective bacteria. This oral mucosal vaccine would provide a useful strategy to substitute the application of antibiotics in the future and induce efficient immune responses against PEDV infection.
Starting Page	1084
e-ISSN	2076393X
DOI	10.3390/vaccines9101084
Journal	Vaccines
Issue Number	10
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2021-09-26
Access Restriction	Open
Subject Keyword	Vaccines Microbiology Lactobacillus Casei Pedv Coe Antigen Alanine Racemase Gene Oral Mucosal Vaccine
Content Type	Text
Resource Type	Article

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