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Characterization of IL-2 Stimulation and TRPM7 Pharmacomodulation in NK Cell Cytotoxicity and Channel Co-Localization with $PIP_{2}$ in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients
| Content Provider | MDPI |
|---|---|
| Author | Preez, Stanley Du Eaton-Fitch, Natalie Cabanas, Helene Staines, Donald Marshall-Gradisnik, Sonya |
| Copyright Year | 2021 |
| Description | Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex multisystemic disorder responsible for significant disability. Although a unifying etiology for ME/CFS is uncertain, impaired natural killer (NK) cell cytotoxicity represents a consistent and measurable feature of this disorder. Research utilizing patient-derived NK cells has implicated dysregulated calcium $(Ca^{2+}$) signaling, dysfunction of the phosphatidylinositol-4,5-bisphosphate $(PIP_{2}$)-dependent cation channel, transient receptor potential melastatin (TRPM) 3, as well as altered surface expression patterns of TRPM3 and TRPM2 in the pathophysiology of ME/CFS. TRPM7 is a related channel that is modulated by $PIP_{2}$ and participates in $Ca^{2+}$ signaling. Though TRPM7 is expressed on NK cells, the role of TRPM7 with IL-2 and intracellular signaling mechanisms in the NK cells of ME/CFS patients is unknown. This study examined the effect of IL-2 stimulation and TRPM7 pharmacomodulation on NK cell cytotoxicity using flow cytometric assays as well as co-localization of TRPM7 with $PIP_{2}$ and cortical actin using confocal microscopy in 17 ME/CFS patients and 17 age- and sex-matched healthy controls. The outcomes of this investigation are preliminary and indicate that crosstalk between IL-2 and TRMP7 exists. A larger sample size to confirm these findings and characterization of TRPM7 in ME/CFS using other experimental modalities are warranted. |
| Starting Page | 11879 |
| e-ISSN | 16604601 |
| DOI | 10.3390/ijerph182211879 |
| Journal | International Journal of Environmental Research and Public Health |
| Issue Number | 22 |
| Volume Number | 18 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-11-12 |
| Access Restriction | Open |
| Subject Keyword | International Journal of Environmental Research and Public Health Immunology Myalgic Encephalomyelitis Chronic Fatigue Syndrome Natural Killer Cell Transient Receptor Potential Melastatin 7 Il-2 Pip2 |
| Content Type | Text |
| Resource Type | Article |
