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The Biolog EcoPlate™ Technique for Assessing the Effect of Metal Oxide Nanoparticles on Freshwater Microbial Communities
| Content Provider | MDPI |
|---|---|
| Author | Imre, Németh Szabina, Molnár Molnár, Mónika Vaszita, Emese |
| Copyright Year | 2021 |
| Description | The application of Biolog EcoPlate™ for community-level physiological profiling of soils is well documented; however, the functional diversity of aquatic bacterial communities has been hardly studied. The objective of this study was to investigate the applicability of the Biolog EcoPlate™ technique and evaluate comparatively the applied endpoints, for the characterisation of the effects of metal oxide nanoparticles (MONPs) on freshwater microbial communities. Microcosm experiments were run to assess the effect of nano ZnO and nano $TiO_{2}$ in freshwater at 0.8–100 mg/L concentration range. The average well colour development, substrate average well colour development, substrate richness, Shannon index and evenness, Simpson index, McIntosh index and Gini coefficient were determined to quantify the metabolic capabilities and functional diversity. Comprehensive analysis of the experimental data demonstrated that short-term exposure to $TiO_{2}$ and ZnO NPs affected the metabolic activity at different extent and through different mechanisms of action. $TiO_{2}$ NPs displayed lower impact on the metabolic profile showing up to 30% inhibition. However, the inhibitory effect of ZnO NPs reached 99% with clearly concentration-dependent responses. This study demonstrated that the McIntosh and Gini coefficients were well applicable and sensitive diversity indices. The parallel use of general metabolic capabilities and functional diversity indices may improve the output information of the ecological studies on microbial communities. |
| Starting Page | 1777 |
| e-ISSN | 20794991 |
| DOI | 10.3390/nano11071777 |
| Journal | Nanomaterials |
| Issue Number | 7 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-07-08 |
| Access Restriction | Open |
| Subject Keyword | Nanomaterials Water Science and Technology Biolog Ecoplate™ Freshwater Microbial Community Biodiversity Metal Oxide Nanoparticles Nano Titanium Dioxide Nano Zinc Oxide |
| Content Type | Text |
| Resource Type | Article |
