NDLI: A Design of Experiment Approach to Optimize Spray-Dried Powders Containing Pseudomonas aeruginosaPodoviridae and Myoviridae Bacteriophages

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A Design of Experiment Approach to Optimize Spray-Dried Powders Containing Pseudomonas aeruginosaPodoviridae and Myoviridae Bacteriophages

Content Provider	MDPI
Author	Tabare, Emilie Glonti, Tea Cochez, Christel Ngassam, Cyrille Pirnay, Jean-Paul Amighi, Karim Goole, Jonathan
Copyright Year	2021
Description	In the present study, we evaluated the effect of spray-drying formulations and operating parameters of a laboratory-scale spray-dryer on the characteristics of spray-dried powders containing two Pseudomonas aeruginosa bacteriophages exhibiting different morphotypes: a podovirus (LUZ19) and a myovirus (14-1). We optimized the production process for bacteriophage-loaded powders, with an emphasis on long-term storage under ICH (international conference on harmonization) conditions. D-trehalose-/L-isoleucine-containing bacteriophage mixtures were spray-dried from aqueous solutions using a Büchi Mini Spray-dryer B-290 (Flawil, Switzerland). A response surface methodology was used for the optimization of the spray-drying process, with the following as-evaluated parameters: Inlet temperature, spray gas flow rate, and the D-trehalose/L-isoleucine ratio. The dried powders were characterized in terms of yield, residual moisture content, and bacteriophage lytic activity. L-isoleucine has demonstrated a positive impact on the activity of LUZ19, but a negative impact on 14-1. We observed a negligible impact of the inlet temperature and a positive correlation of the spray gas flow rate with bacteriophage activity. After optimization, we were able to obtain dry powder preparations of both bacteriophages, which were stable for a minimum of one year under different ICH storage conditions (up to and including 40 °C and 75% relative humidity).
Starting Page	1926
e-ISSN	19994915
DOI	10.3390/v13101926
Journal	Viruses
Issue Number	10
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-09-24
Access Restriction	Open
Subject Keyword	Viruses Pharmacology and Pharmacy Doe Qbd Antibiotic-resistant Bacteriophage Therapy Spray-drying Drying Stability Pseudomonas Aeruginosa Luz19 14-1
Content Type	Text
Resource Type	Article

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