NDLI: Advances in Lipid Extraction Methods—A Review

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Advances in Lipid Extraction Methods—A Review

Content Provider	MDPI
Author	Saini, Ramesh Kumar Prasad, Parchuri Shang, Xiaomin Keum, Young-Soo
Copyright Year	2021
Description	Extraction of lipids from biological tissues is a crucial step in lipid analysis. The selection of appropriate solvent is the most critical factor in the efficient extraction of lipids. A mixture of polar (to disrupt the protein-lipid complexes) and nonpolar (to dissolve the neutral lipids) solvents are precisely selected to extract lipids efficiently. In addition, the disintegration of complex and rigid cell-wall of plants, fungi, and microalgal cells by various mechanical, chemical, and enzymatic treatments facilitate the solvent penetration and extraction of lipids. This review discusses the chloroform/methanol-based classical lipid extraction methods and modern modifications of these methods in terms of using healthy and environmentally safe solvents and rapid single-step extraction. At the same time, some adaptations were made to recover the specific lipids. In addition, the high throughput lipid extraction methodologies used for liquid chromatography-mass spectrometry (LC-MS)-based plant and animal lipidomics were discussed. The advantages and disadvantages of various pretreatments and extraction methods were also illustrated. Moreover, the emerging green solvents-based lipid extraction method, including supercritical $CO_{2}$ extraction (SCE), is also discussed.
Starting Page	13643
e-ISSN	14220067
DOI	10.3390/ijms222413643
Journal	International Journal of Molecular Sciences
Issue Number	24
Volume Number	22
Language	English
Publisher	MDPI
Publisher Date	2021-12-20
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Analytical Chemistry Lipidomics Folch Method Bligh and Dyer Method Soxhlet Extraction Supercritical Co2 Extraction Pre-treatments Green Solvents
Content Type	Text
Resource Type	Article

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