NDLI: Genome-Wide Identification of Cotton (Gossypium spp.) Glycerol-3-Phosphate Dehydrogenase (GPDH) Family Members and the Role of GhGPDH5 in Response to Drought Stress

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Genome-Wide Identification of Cotton (Gossypium spp.) Glycerol-3-Phosphate Dehydrogenase (GPDH) Family Members and the Role of GhGPDH5 in Response to Drought Stress

Content Provider	MDPI
Author	Sun, Jialiang Cui, Hua Wu, Bingjie Wang, Weipeng Yang, Qiuyue Zhang, Yaxin Yang, Song Zhao, Yuping Xu, Dongbei Liu, Guoxiang Qin, Tengfei
Copyright Year	2022
Description	Glycerol-3-phosphate dehydrogenase (GPDH) is a key enzyme in plant glycerol synthesis and metabolism, and plays an important role in plant resistance to abiotic stress. Here, we identified 6, 7, 14 and 14 GPDH genes derived from Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively. Phylogenetic analysis assigned these genes into three classes, and most of the genes within the family were expanded by whole-genome duplication (WGD) and segmental duplications. Moreover, determination of the nonsynonymous substitution rate/synonymous substitution rate (Ka/Ks) ratio showed that the GPDH had an evolutionary preference for purifying selection. Transcriptome data revealed that GPDH genes were more active in the early stages of fiber development. Additionally, numerous stress-related cis-elements were identified in the potential promoter region. Then, a protein–protein-interaction (PPI) network of GPDH5 in G. hirsutum was constructed. In addition, we predicted 30 underlying miRNAs in G. hirsutum. Functional validation results indicated that silencing GhGPDH5 diminished drought tolerance in the upland cotton TM-1 line. In summary, this study provides a fundamental understanding of the GPDH gene family in cotton, GhGPDH5 exerts a positive effect during drought stress and is potentially involved in stomatal closure movements.
Starting Page	592
e-ISSN	22237747
DOI	10.3390/plants11050592
Journal	Plants
Issue Number	5
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2022-02-22
Access Restriction	Open
Subject Keyword	Plants Plant Sciences Drought Stress Replication Events Transcriptional Expression Profiles Protein-protein Interaction Network Stomatal Aperture
Content Type	Text
Resource Type	Article

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