NDLI: Biological and Cytoprotective Effect of Piper kadsura Ohwi against Hydrogen-Peroxide-Induced Oxidative Stress in Human SW1353 Cells

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Biological and Cytoprotective Effect of Piper kadsura Ohwi against Hydrogen-Peroxide-Induced Oxidative Stress in Human SW1353 Cells

Content Provider	MDPI
Author	Huang, Te-Yang Wu, Chih-Chuan Su, Wen-Ta
Copyright Year	2021
Description	Oxidative stress plays a role in regulating a variety of physiological functions in living organisms and in the pathogenesis of articular cartilage diseases. Piper kadsura Ohwi is a traditional Chinese medicine that is used as a treatment for rheumatic pain, and the extracts have anti-inflammatory and antioxidant effects. However, there is still no study related to cell protection by P. kadsura. The P. kadsura extracts (PKE) were obtained by microwave-assisted extraction, liquid-liquid extraction, and column chromatography separation. The extracts could effectively scavenge free radicals in the antioxidant test, the $EC_{50}$ of extracts is approximately the same as vitamin C. PKE decreased the apoptosis of SW1353 cells treated with $H_{2}O_{2}$ and could upregulate the gene expression of antioxidant enzymes (SOD-2, GPx, and CAT) and the Bcl-2/Bax ratio, as well as regulate PARP, thus conferring resistance to $H_{2}O_{2}$ attack. PKE protects cells against apoptosis caused by free radicals through the three pathways of JNK, MEK/ERK, and p38 by treatment with MAPK inhibitor. The identified components of PKE were bicyclo [2.2.1] heptan-2-ol-1,7,7-trimethyl-,(1S-endo)-, alpha-humulene, and hydroxychavicol by gas chromatography–mass spectrometry.
Starting Page	6287
e-ISSN	14203049
DOI	10.3390/molecules26206287
Journal	Molecules
Issue Number	20
Volume Number	26
Language	English
Publisher	MDPI
Publisher Date	2021-10-18
Access Restriction	Open
Subject Keyword	Molecules Integrative and Complementary Medicine Piper Kadsura Ohwi Microwave-assisted Extraction Oxidative Stress Sw1353 Mapks Pathways
Content Type	Text
Resource Type	Article

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