NDLI: Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of Ammodaucus leucotrichus. Untargeted Metabolomics Analysis by UHPLC-MS/MS

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Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of Ammodaucus leucotrichus. Untargeted Metabolomics Analysis by UHPLC-MS/MS

Content Provider	MDPI
Author	Jose, David Sánchez-Martínez Abderrezag, Norelhouda Louaer, Ouahida Meniai, Abdeslam-Hassen Mendiola, Jose A.
Copyright Year	2021
Description	Ammodaucus leucotrichus is a spontaneous plant endemic of the North African region. An efficient selective pressurized liquid extraction (PLE) method was optimized to concentrate neuroprotective extracts from A. leucotrichus fruits. Green solvents were tested, namely ethanol and water, within a range of temperatures between 40 to 180 °C. Total carbohydrates and total phenolics were measured in extracts, as well as in vitro antioxidant capacity (DPPH radical scavenging), anticholinesterase (AChE) and anti-inflammatory (LOX) activities. Metabolite profiling was carried out by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-q-TOF-MS/MS), identifying 94 compounds. Multivariate analysis was performed to correlate composition with bioactivity. A remarkable effect of the temperature using water was observed: the higher temperature, the higher extraction yield, the higher total phenolic content, as well as the higher total carbohydrates content. The water extract obtained at 180 °C, 10.34 MPa and 10 min showed meaningful anti-inflammatory $(IC50_{LOX}$ = 39.4 µg/mL) and neuroprotective activities $(IC50_{AChE}$ = 55.6 µg/mL). The Principal Components Analysis (PCA) and the cluster analysis correlated these activities with the presence of carbohydrates and phenolic compounds.
Starting Page	6951
e-ISSN	14203049
DOI	10.3390/molecules26226951
Journal	Molecules
Issue Number	22
Volume Number	26
Language	English
Publisher	MDPI
Publisher Date	2021-11-17
Access Restriction	Open
Subject Keyword	Molecules Biochemical Research Ammodaucus Leucotrichus Bioprospecting Pressurized Liquid Extraction Uhplc-q-tof-ms/ms Neuroprotective Potential Anti-inflammatory Activity
Content Type	Text
Resource Type	Article

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