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Design and Clinical Application of an Integrated Microfluidic Device for Circulating Tumor Cells Isolation and Single-Cell Analysis
| Content Provider | MDPI |
|---|---|
| Author | Xu, Mingxin Liu, Wenwen Zou, Kun Wei, Song Zhang, Xinri Li, Encheng Wang, Qi |
| Copyright Year | 2021 |
| Description | Circulating tumor cells (CTCs) have been considered as an alternative to tissue biopsy for providing both germline-specific and tumor-derived genetic variations. Single-cell analysis of CTCs enables in-depth investigation of tumor heterogeneity and individualized clinical assessment. However, common CTC enrichment techniques generally have limitations of low throughput and cell damage. Herein, based on micropore-arrayed filtration membrane and microfluidic chip, we established an integrated CTC isolation platform with high-throughput, high-efficiency, and less cell damage. We observed a capture rate of around 85% and a purity of 60.4% by spiking tumor cells (PC-9) into healthy blood samples. Detection of CTCs from lung cancer patients demonstrated a positive detectable rate of 87.5%. Additionally, single CTCs, ctDNA and liver biopsy tissue of a representative advanced lung cancer patient were collected and sequenced, which revealed comprehensive genetic information of CTCs while reflected the differences in genetic profiles between different biological samples. This work provides a promising tool for CTCs isolation and further analysis at single-cell resolution with potential clinical value. |
| Starting Page | 49 |
| e-ISSN | 2072666X |
| DOI | 10.3390/mi12010049 |
| Journal | Micromachines |
| Issue Number | 1 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-01-02 |
| Access Restriction | Open |
| Subject Keyword | Micromachines Analytical Chemistry Ctc-isolation Microfluidics Single-cell Analysis High-throughput Sequencing Lung Cancer |
| Content Type | Text |
| Resource Type | Article |
