NDLI: Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds

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Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds

Content Provider	MDPI
Author	Wang, Hsueh-Chun Lin, Tzu-Hsiang Hsu, Che-Chia Yeh, Ming-Long
Copyright Year	2021
Description	Cartilage stem/progenitor cells (CSPCs) are cartilage-specific, multipotent progenitor cells residing in articular cartilage. In this study, we investigated the characteristics and potential of human CSPCs combined with poly(lactic-co-glycolic acid) (PLGA) scaffolds to induce osteochondral regeneration in rabbit knees. We isolated CSPCs from human adult articular cartilage undergoing total knee replacement (TKR) surgery. We characterized CSPCs and compared them with infrapatellar fat pad-derived stem cells (IFPs) in a colony formation assay and by multilineage differentiation analysis in vitro. We further evaluated the osteochondral regeneration of the CSPC-loaded PLGA scaffold during osteochondral defect repair in rabbits. The characteristics of CSPCs were similar to those of mesenchymal stem cells (MSCs) and exhibited chondrogenic and osteogenic phenotypes without chemical induction. For in vivo analysis, CSPC-loaded PLGA scaffolds produced a hyaline-like cartilaginous tissue, which showed good integration with the host tissue and subchondral bone. Furthermore, CSPCs migrated in response to injury to promote subchondral bone regeneration. Overall, we demonstrated that CSPCs can promote osteochondral regeneration. A monophasic approach of using diseased CSPCs combined with a PLGA scaffold may be beneficial for repairing complex tissues, such as osteochondral tissue.
Starting Page	3536
e-ISSN	20734409
DOI	10.3390/cells10123536
Journal	Cells
Issue Number	12
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-12-14
Access Restriction	Open
Subject Keyword	Cells Cell Tissue Engineering Osteochondral Tissue Engineering Cartilage Stem/progenitor Cell Poly (lactic-co-glycolic Acid) Scaffold Migration Monophasic Approach
Content Type	Text
Resource Type	Article

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