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25-Hydroxycholesterol Effect on Membrane Structure and Mechanical Properties
| Content Provider | MDPI |
|---|---|
| Author | Domingues, Marco Gomes, Bárbara Hollmann, Axel Santos, Nuno |
| Copyright Year | 2021 |
| Description | Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with several viruses’ entry into their target cells. We used atomic force microscopy to assess the effect of 25-hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25-hydroxycholesterol inhibits the lipid-condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25-hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25-hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)-enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations. |
| Starting Page | 2574 |
| e-ISSN | 14220067 |
| DOI | 10.3390/ijms22052574 |
| Journal | International Journal of Molecular Sciences |
| Issue Number | 5 |
| Volume Number | 22 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-03-04 |
| Access Restriction | Open |
| Subject Keyword | International Journal of Molecular Sciences Biophysics Cholesterol 25-hydroxycholesterol Atomic Force Microscopy Force Spectroscopy Supported Lipid Bilayers |
| Content Type | Text |
| Resource Type | Article |
