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Graphene Bioelectronic Nose for the Detection of Odorants with Human Olfactory Receptor 2AG1
| Content Provider | MDPI |
|---|---|
| Author | Goodwin, Danielle Walters, Ffion Ali, Muhammad Ahmadi, Ehsaneh Daghigh Guy, Owen |
| Copyright Year | 2021 |
| Description | A real-time sensor for the detection of amyl butyrate (AB) utilising human olfactory receptor 2AG1 (OR2AG1), a G-protein coupled receptor (GPCR) consisting of seven transmembrane domains, immobilized onto a graphene resistor is demonstrated. Using CVD graphene as the sensor platform, allows greater potential for more sensitive detection than similar sensors based on carbon nanotubes, gold or graphene oxide platforms. A specific graphene resistor sensor was fabricated and modified via non-covalent π–π stacking of 1,5 diaminonaphthalene (DAN) onto the graphene channel, and subsequent anchoring of the OR2AG1 receptor to the DAN molecule using glutaraldehyde coupling. Binding between the target odorant, amyl butyrate, and the OR2AG1 receptor protein generated a change in resistance of the graphene resistor sensor. The functionalized graphene resistor sensors exhibited a linear sensor response between 0.1–500 pM and high selectively towards amyl butyrate, with a sensitivity as low as 500 fM, whilst control measurements using non-specific esters, produced a negligible sensor response. The approach described here provides an alternative sensing platform that can be used in bioelectronic nose applications. |
| Starting Page | 174 |
| e-ISSN | 22279040 |
| DOI | 10.3390/chemosensors9070174 |
| Journal | Chemosensors |
| Issue Number | 7 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-07-08 |
| Access Restriction | Open |
| Subject Keyword | Chemosensors Analytical Chemistry Real-time Sensor Π–π Stacking Drop-cast Carbon Surfaces Resistor Passivated Mvd Non-covalent |
| Content Type | Text |
| Resource Type | Article |
