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DNA–Gold Nanozyme-Modified Paper Device for Enhanced Colorimetric Detection of Mercury Ions
| Content Provider | MDPI |
|---|---|
| Author | Mao, Min-Xin Zheng, Rong Peng, Chi-Fang Wei, Xin-Lin |
| Copyright Year | 2020 |
| Description | In this work, a paper device consisted of a patterned paper chip, wicking pads, and a base was fabricated. On the paper chip, DNA–gold nanoparticles (DNA–AuNPs) were deposited and Hg2+ ions could be adsorbed by the DNA–AuNPs. The formed DNA–AuNP/Hg2+ nanozyme could catalyze the tetramethylbenzidine (TMB) –H2O2 chromogenic reaction. Due to the wicking pads, a larger volume of Hg2+ sample could be applied to the paper device for Hg2+ detection and therefore the color response could be enhanced. The paper device achieved a cut-off value of 50 nM by the naked eye for Hg2+ under optimized conditions. Moreover, quantitative measurements could be implemented by using a desktop scanner and extracting grayscale values. A linear range of 50–2000 nM Hg2+ was obtained with a detection limit of 10 nM. In addition, the paper device could be applied in the detection of environmental water samples with high recoveries ranging from 85.7% to 105.6%. The paper-device-based colorimetric detection was low-cost, simple, and demonstrated high potential in real-sample applications. |
| Starting Page | 211 |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios10120211 |
| Journal | Biosensors |
| Issue Number | 12 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-12-18 |
| Access Restriction | Open |
| Subject Keyword | Biosensors Analytical Chemistry Paper Device Signal Enhancement Mercury Ion Colorimetric Detection |
| Content Type | Text |
| Resource Type | Article |
