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Ethanol Production from High Solid Loading of Rice Straw by Simultaneous Saccharification and Fermentation in a Non-Conventional Reactor
| Content Provider | MDPI |
|---|---|
| Author | Roberto, Inês C. Castro, Rafael C. A. Silva, João Paulo A. Mussatto, Solange I. |
| Copyright Year | 2020 |
| Description | Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approach to improve the economic feasibility of cellulosic ethanol. In this study, SSF using high loading of rice straw was assessed using a vertical ball mill reactor. First, the conditions of temperature and number of glass spheres were optimized at 8% (w/v) initial solids (41.5 °C, 18 spheres). Then, assays were carried out at higher solid loadings (16% and 24% w/v). At 8% or 16% solids, the fermentation efficiency was similar (ηF~75%), but the ethanol volumetric productivity (QP) reduced from 1.50 to 1.14 g/L.h. By increasing the solids to 24%, the process was strongly affected (ηF = 40% and QP = 0.7 g/L.h). To overcome this drawback, three different feeding profiles of 24% pre-treated rice straw were investigated. Gradual feeding of the substrate (initial load of 16% with additions of 4% at 10 and 24 h) and an inoculum level of 3 g/L resulted in a high ethanol titer (52.3 g/L) with QP of 1.1 g/L.h and ηF of 67%. These findings demonstrated that using a suitable fed-batch feeding strategy helps to overcome the limitations of SSF in batch mode caused by the use of high solid content. |
| Starting Page | 2090 |
| e-ISSN | 19961073 |
| DOI | 10.3390/en13082090 |
| Journal | Energies |
| Issue Number | 8 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-04-22 |
| Access Restriction | Open |
| Subject Keyword | Energies Paper and Wood Ethanol Simultaneous Saccharification and Fermentation Rice Straw Vertical Ball Mill Reactor Kluyveromyces Marxianus |
| Content Type | Text |
| Resource Type | Article |