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Toward Cancer Diagnostics of the Tumor Suppressor p53 by Surface Enhanced Raman Spectroscopy
| Content Provider | MDPI |
|---|---|
| Author | Bizzarri, Anna Rita Cannistraro, Salvatore |
| Copyright Year | 2020 |
| Description | The tumor suppressor p53 protein plays a crucial role in many biological processes. The presence of abnormal concentrations of wild-type p53, or some of its mutants, can be indicative of a pathological cancer state. p53 represents therefore a valuable biomarker for tumor screening approaches and development of suitable biosensors for its detection deserves a high interest in early diagnostics. Here, we revisit our experimental approaches, combining Surface Enhanced Raman Spectroscopy (SERS) and nanotechnological materials, for ultrasensitive detection of wild-type and mutated p53, in the perspective to develop biosensors to be used in clinical diagnostics. The Raman marker is provided by a small molecule (4-ATP) acting as a bridge between gold nanoparticles (NPs) and a protein biomolecule. The Azurin copper protein and specific antibodies of p53 were used as a capture element for p53 (wild-type and its mutants). The developed approaches allowed us to reach a detection level of p53 down to 10−17 M in both buffer and serum. The implementation of the method in a biosensor device, together with some possible developments are discussed. |
| Starting Page | 7153 |
| e-ISSN | 14248220 |
| DOI | 10.3390/s20247153 |
| Journal | Sensors |
| Issue Number | 24 |
| Volume Number | 20 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-12-14 |
| Access Restriction | Open |
| Subject Keyword | Sensors Analytical Chemistry Surface Enhanced Raman Spectroscopy (sers) P53 P53 Mutants Ultrasensitive Detection Cancer Biomarkers |
| Content Type | Text |
| Resource Type | Article |
