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Synergistic Effects of Plant Growth Regulators and Elicitors on α-Humulene and Zerumbone Production in Zingiber zerumbet Smith Adventitious Root Cultures
| Content Provider | MDPI |
|---|---|
| Author | Alwakil, Nurul Huda Annuar, Mohamad Suffian Mohamad Jalil, Mahanom |
| Copyright Year | 2022 |
| Description | Zingiber zerumbet, also known as ‘Lempoyang’, possesses various phytomedicinal properties, such as anticancer, antimicrobial, anti-inflammatory, antiulcer, and antioxidant properties. Secondary metabolites possessing such properties i.e., zerumbone and α-humulene, are found dominantly in the plant rhizome. Synergistic effects of plant growth hormones and elicitors on in vitro α-humulene and zerumbone production, and biomass growth, in adventitious root culture (AdRC) of Z. zerumbet cultivated in a two-stage culture are reported. The culture was induced by supplementation of 1.0 mg/L NAA and 2.0 mg/L IBA (dark), and subsequently maintained in medium supplemented with 1 mg/L NAA and 3 mg/L BAP (16:08 light-dark cycle), yielded the production of zerumbone at 3440 ± 168 µg/g and α-humulene at 3759 ± 798 µg/g. Synergistic elicitation by 400 μM methyl jasmonate (MeJa) and 400 μM salicylic acid (SA) resulted in a 13-fold increase in zerumbone (43,000 ± 200 µg/g), while 400 μM MeJa and 600 μM SA produced a 4.3-fold increase in α-humulene (15,800 ± 5100 µg/g) compared to control. |
| Starting Page | 4744 |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules27154744 |
| Journal | Molecules |
| Issue Number | 15 |
| Volume Number | 27 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-07-25 |
| Access Restriction | Open |
| Subject Keyword | Molecules Horticulture Plant Sciences Phytomedicinal Secondary Metabolites in Vitro Methyl Jasmonate Salicylic Acid |
| Content Type | Text |
| Resource Type | Article |
