Loading...
Please wait, while we are loading the content...
Similar Documents
Easy-to-Operate Co-Flow Step Emulsification Device for High-Throughput Three-Dimensional Cell Culture
Content Provider | MDPI |
---|---|
Author | Wei, Chunyang Yu, Chengzhuang Li, Shanshan Li, Tiejun Meng, Jiyu Li, Junwei |
Copyright Year | 2022 |
Description | Cell culture plays an essential role in tissue engineering and high-throughput drug screening. Compared with two-dimensional (2D) in vitro culture, three-dimensional (3D) in vitro culture can mimic cells in vivo more accurately, including complex cellular organizations, heterogeneity, and cell–extracellular matrix (ECM) interactions. This article presents a droplet-based microfluidic chip that integrates cell distribution, 3D in vitro cell culture, and in situ cell monitoring in a single device. Using the microfluidic “co-flow step emulsification” approach, we have successfully prepared close-packed droplet arrays with an ultra-high-volume fraction (72%) which can prevent cells from adhering to the chip surface so as to achieve a 3D cell culture and make scalable and high-throughput cell culture possible. The proposed device could produce droplets from 55.29 ± 1.52 to 95.64 ± 3.35 μm, enabling the diverse encapsulation of cells of different sizes and quantities. Furthermore, the cost for each microfluidic CFSE chip is approximately USD 3, making it a low-cost approach for 3D cell culture. The proposed device is successfully applied in the 3D culture of saccharomyces cerevisiae cells with an occurrence rate for proliferation of 80.34 ± 3.77%. With low-cost, easy-to-operate, high-throughput, and miniaturization characteristics, the proposed device meets the requirements for 3D in vitro cell culture and is expected to be applied in biological fields such as drug toxicology and pharmacokinetics. |
Starting Page | 350 |
e-ISSN | 20796374 |
DOI | 10.3390/bios12050350 |
Journal | Biosensors |
Issue Number | 5 |
Volume Number | 12 |
Language | English |
Publisher | MDPI |
Publisher Date | 2022-05-18 |
Access Restriction | Open |
Subject Keyword | Biosensors Analytical Chemistry Microfluidics Droplet Arrays 3d Cell Culture Co-flow Step Emulsification Lab On a Chip |
Content Type | Text |
Resource Type | Article |