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Identification and Characterization of a Novel Endo-β-1,4-Xylanase from Streptomyces sp. T7 and Its Application in Xylo-Oligosaccharide Production
| Content Provider | MDPI |
|---|---|
| Author | Li, Yumei Zhang, Xinxin Lu, Chunwen Lu, Peng Yin, Chongxu Ye, Zhengmao Huang, Zhaosong |
| Copyright Year | 2022 |
| Description | A xylanase-producing strain, identified as Streptomyces sp. T7, was isolated from soil by our lab. The endo-β-1,4-xylanase (xynST7) gene was found in the genome sequence of strain T7, which was cloned and expressed in Escherichia coli. XynST7 belonged to the glycoside hydrolase family 10, with a molecular mass of approximately 47 kDa. The optimum pH and temperature of XynST7 were pH 6.0 and 60 °C, respectively, and it showed wide pH and temperature adaptability and stability, retaining more than half of its enzyme activity between pH 5.0 and 11.0 below 80 °C. XynST7 showed only endo-β-1,4-xylanase activity without cellulase- or β-xylosidase activity, and it showed maximal hydrolysis for corncob xylan in all the test substrates. Then, XynST7 was used for the production of xylo-oligosaccharides (XOSs) by hydrolyzing xylan extracted from raw corncobs. The maximum yield of the XOS was 8.61 ± 0.13 mg/mL using 15 U/mL of XynST7 and 1.5% corncob xylan after 10 h of incubation at 60 °C. The resulting hydrolysate products mainly consisted of xylobiose and xylotriose. These data indicated that XynST7 might by a promising tool for various industrial applications. |
| Starting Page | 2516 |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules27082516 |
| Journal | Molecules |
| Issue Number | 8 |
| Volume Number | 27 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-04-13 |
| Access Restriction | Open |
| Subject Keyword | Molecules Microbiology Endo-β-1,4-xylanase Expression Xylo-oligosaccharides |
| Content Type | Text |
| Resource Type | Article |
