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Expression and Characterization of a Novel Cold-Adapted and Stable β-Agarase Gene agaW1540 from the Deep-Sea Bacterium Shewanella sp. WPAGA9
| Content Provider | MDPI |
|---|---|
| Author | Wang, Wenxin Wang, Jianxin Yan, Ruihua Zeng, Runying Zuo, Yaqiang Wang, Dingquan Qu, Wu |
| Copyright Year | 2021 |
| Description | The neoagaro-oligosaccharides, degraded from agarose by agarases, are important natural substances with many bioactivities. In this study, a novel agarase gene, agaW1540, from the genome of a deep-sea bacterium Shewanella sp. WPAGA9, was expressed, and the recombinant AgaW1540 (rAgaW1540) displayed the maximum activity under the optimal pH and temperature of 7.0 and 35 °C, respectively. rAgaW1540 retained 85.4% of its maximum activity at 0 °C and retained more than 92% of its maximum activity at the temperature range of 20–40 °C and the pH range of 4.0–9.0, respectively, indicating its extensive working temperature and pH values. The activity of rAgaW1540 was dramatically suppressed by $Cu^{2+}$ and $Zn^{2+}$, whereas $Fe^{2+}$ displayed an intensification of enzymatic activity. The $K_{m}$ and $V_{max}$ of rAgaW1540 for agarose degradation were 15.7 mg/mL and 23.4 U/mg, respectively. rAgaW1540 retained 94.7%, 97.9%, and 42.4% of its maximum activity after incubation at 20 °C, 25 °C, and 30 °C for 60 min, respectively. Thin-layer chromatography and ion chromatography analyses verified that rAgaW1540 is an endo-acting β-agarase that degrades agarose into neoagarotetraose and neoagarohexaose as the main products. The wide variety of working conditions and stable activity at room temperatures make rAgaW1540an appropriate bio-tool for further industrial production of neoagaro-oligosaccharides. |
| Starting Page | 431 |
| e-ISSN | 16603397 |
| DOI | 10.3390/md19080431 |
| Journal | Marine drugs |
| Issue Number | 8 |
| Volume Number | 19 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-07-29 |
| Access Restriction | Open |
| Subject Keyword | Marine drugs Marine Drugs Microbiology Neoagaro-oligosaccharides Β-agarase Cold-adapted Stable Deep-sea |
| Content Type | Text |
| Resource Type | Article |
